Inhibition of tumor immune escape by blocking PD-1/PD-L1 engagement with dual-targeting molecularly imprinted polymer layer

Abstract Blocking the binding of PD-1/PD-L1 has become an effective strategy in inhibition tumor immune escape. At present, it mainly depends on employment macromolecular antibodies, which target protein through one PD-1 or PD-L1 domains. In this study, we present a different strategy, aptamer modified molecularly imprinted polymer layer (APD–PD-L1–MIPL), to break for The APD–PD-L1–MIPL is prepared by MIP surface CaCO 3 nanospheres using peptide segment as template. subsequent removal core formats layer, ensure high specifically matching capacity and short equilibrium time. A antagonistic DNA aptamer, into enhance recognition capacity, resulting dual-targeting functionality. able bind allow suppressing engagement with PD-1, inducing block downstream signaling pathways and, therefore, restore T cell function cancer growth. can quantitatively detect bound proteins LOD 0.003 mg mL −1 . This enables provide new idea immunotherapy.